Dr. Reza Yousefi

 

                                   
Reza Yousefi
Professor of Biochemistry
Tel: +98-21 6111-3378/66956978  
E-mail:yousefi.reza@ut.ac.ir  
              

     Reza Yousefi was born in 1972 in Ramian, Gorgan (northern Iran) and completed his education from elementary school to high school in his hometown. In 1996, he graduated in bachelor's degree of biology from Shiraz University, and in the same year, he joined Institute of Biochemistry and Biophysics (IBB) of the University of Tehran to continue his education in master and then in PhD of biochemistry.
After a sabbatical period in Switzerland (Bern University) and passing two postdoctoral periods in Iran (IBB) and France (INRA, Nantes), he was employed by Shiraz University in 2009 and was promoted to professor of biochemistry at this university in 2018. In addition to teaching biochemistry and advanced topics on the structure and function of proteins, his research interests are mainly focused on the application of the recombinant DNA technology for expression and production of the disease-associated or therapeutic proteins and peptides and their structural and functional studies. Also, the production of mutant proteins and peptides with the aim of creating improved products or discovering the relationship between mutations and their pathogenicity is another important research interest which is followed by him. Reza Yousefi is also a member of the editorial board of a number of prestigious international journals.             

Link to research laboratory: ibb.ut.ac.ir/en/pcl                                                                                  

Admission of graduate students


The Protein Chemistry Laboratory (PCL) at the University of Tehran accepts master's and doctoral students from top universities who demonstrate exceptional talent. Admission is carried out in a professor-centered manner, with the research project emphasizing the expression and laboratory production of therapeutic proteins and peptides in a bacterial host system, along with their structural and functional analyses. Prospective applicants are required to submit a motivation letter and a CV to the email address provided below and follow the guidelines set forth by the Vice-Chancellor of the University of Tehran.

Email: Yousefi.reza@ut.ac.ir

 

 

Personal Records                                                

  

Education

  
 
Name: Reza Yousefi       
Date of Birth: 10 July 1972             
Place of Birth: Ramian, Gorgan, Iran.           
Marital Status: Married, 2 Children
Appointment: Professor 
Office Address: Protein Chemistry Laboratory (PCL),Institute of Biochemistry and Biophysics, University of Tehran P.O.Box 13145-1384 Tehran,Iran    
Telephone: 0098 21 66956983   
Fax: 0098 21 66404680           
  

BSc in Biology: Department of Biology, Shiraz University, Shiraz, Iran (1996).

MSc in Biochemistry: Institute of Biochemistry and Biophysics (IBB), Tehran University, Tehran, Iran (1999).

PhD in Biochemistry: Institute of Biochemistry and Biophysics (IBB), Tehran University, Tehran, Iran (2005).

Scopus Author ID: 10739992600                     

OrcID: 0000-0001-7396-7720

  

   

 Previous Work History            

  1. Post doctorate research fellow (2005-2006): Institute of Biochemistry and Biophysics (IBB), Tehran University, Tehran, Iran.
  2. Post doctorate research fellow (2006-2008): French National Institute for Agricultural Research (INRA), Nantes, France.
  3. Assistant Professor of Biochemistry at Shiraz University (2009/05/23-2012/05/23).

   Visiting Scholar (February 2005- September 2005):  Department of Chemistry and Biochemistry, Bern University, Bern, Switzerland.

 

 

Research Interest:

Our research group is interested in the role of mutation and chemical modification on structural/functional variation and fibrillation of eye lens crystallins and human insulin. Crystallin proteins are pathogenic targets for various chemical modifications and mutations which ultimately affect the proper function and transparency of eye lens, resulting in cataract and blindness development. Stability and fibrillation of human insulin is currently a big medical and biotechnological challenge. Site-directed mutagenesis is an important technique to obtain new insulin molecules with increased stability and lower propensity for aggregation. We are also planning to produce novel insulin analogs through bioengineered modifications of the natural amino acid sequence of human insulin. We also use DNA recombinant technology, while using new methods and innovations in optimizing the production of pharmaceutical peptides such as those with incretin activity.

Link to research laboratory: ibb.ut.ac.ir/en/pcl    

 

Projects:
A) PhD Project
1- Study of the role of cataract and myopathy associated-mutations on the structure, stability, biological activities and amyloidogenic properties of human recombinant alpha-crystallin. 
2- Production of new insulin analogues by site directed mutagenesis and evaluation of their structure, stability and biological activity.
3- Optimization of expression and production of peptides with therapeutic applications in the bacterial host system.
 
B) MSc Project
1) Development of novel anti-diabetic compounds with inhibitory action against alpha-glucosidase and alpha-amylase enzymes.
2) Anticancer activity assessments of synthetic and natural compounds.

 

  • Barati, A., Rezaei Somee, L., Shahsavani, M. B., Ghasemi, A., Hoshino, M., Hong, J., ... & Yousefi, R. (2024). Insights into the dual nature of αB-crystallin chaperone activity from the p. P39L mutant at the N-terminal region. Scientific Reports, 14(1), 7353.
  • Nasiri, F., Ebrahimi, P., Shahsavani, M. B., Barati, A., Zarei, I., Hong, J., ... & Yousefi, R. (2024). Unraveling the impact of the p. R107L mutation on the structure and function of human αB-Crystallin: Implications for cataract formation. Biochimie, 222, 151-168.
  • Moosavi-Movahedi F, Saboury AA, Ghasemi A, Pirhaghi M, Mamashli F, Mohammad-Zaheri M, Arghavani P, Yousefi R, Moosavi-Movahedi AA. Exploring the significance of potassium homeostasis in copper ion binding to human αB-Crystallin. International Journal of Biological Macromolecules. 2024 Feb 16:130261.
  • Latifi A, Hosseini E, Daneshgar H, Edrisi M, Arghavani P, Bagherzadeh M, Yousefi R, Moosavi-Movahedi AA. Fibril-Droplet Relationship Through Liquid-Liquid Phase Separation; A BSA-MOF Case.
  • Shahsavani MB, Hoshino M, Kumar A, Yousefi R. Charge manipulation of the human insulin B chain C-terminal to shed light on the complex mechanism of insulin fibrillation. Biochimica et Biophysica Acta (BBA)-General Subjects. 2024 Apr 1;1868(4):130578.
  • Somee LR, Barati A, Shahsavani MB, Hoshino M, Hong J, Kumar A, Moosavi-Movahedi AA, Amanlou M, Yousefi R. Understanding the structural and functional changes and biochemical pathomechanism of the cardiomyopathy-associated p. R123W mutation in human αB-crystallin. Biochimica et Biophysica Acta (BBA)-General Subjects. 2024 Apr 1;1868(4):130579.
  • Albaghlany RM, Shahsavani MB, Hoshino M, Moosavi-Movahedi AA, Ghasemi Y, Yousefi R. Optimizing expression, purification, structural and functional assessments of a novel dimeric incretin (GLP-1cpGLP-1). Biochimie. 2023 Nov 4:S0300-9084(23)00291-2. doi: 10.1016/j.biochi.2023.10.020. Epub ahead of print. PMID: 37931794.
  • Hosseini Jafari M, Shahsavani MB, Hoshino M, Hong J, Saboury AA, Moosavi-Movahedi AA, Yousefi R. Unveiling the structural and functional consequences of the p.D109G pathogenic mutation in human αB-Crystallin responsible for restrictive cardiomyopathy and skeletal myopathy. Int J Biol Macromol. 2023 Nov 7;254(Pt 3):127933. doi: 10.1016/j.ijbiomac.2023.127933. Epub ahead of print. PMID: 37939764.
  • Shojai S, Meftahi M, Yousefi R, Moosavi-Movahedi AA, Haeri-Rohani A, Habibi-Rezaei M. Cellulose membrane coated Mo-doped TiO2nanotube sheets for sustained oxidation of biomolecules. Nanotechnology. 2023 May 19;34(31). doi: 10.1088/1361-6528/acd258. PMID: 37141862.
  • Ghahramani M,  Shahsavani M.B, Yousefi R, Increased chaperone activity of human α‌B-crystallin with incomplete oxidation as a new defense mechanism against oxidative stress, Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, 2022 May 26;1870(7):140794. doi: 10.1016/j.bbapap.2022.140794.
  • Ahmadi S, Shahsavani M.B, Tavaf Z, Albaghlani R.M, Kumar A, Moosavi-Movahedi A.A, Yousefi R,  A novel strategy ‌for production of liraglutide precursor peptide and development of a new long-acting incretin mimic, PLOS ONE, 2022, https://doi.org/10.1371/journal.pone.0266833
  •  Ghanbarnezhad M.M, Shahsavani M.M, Mali P.S, Upadhyay M., Kumar A, Albaghlani R.M, Niazi A, Yousefi R, Developing a novel exenatide-based incretin mimic (αB-Ex): Expression, purification and structural-functional characterization, Biochimica et Biophysica Acta (BBA)- General Subjects, 2022, doi: 10.1016/j.bbagen.2022.130150.
  • Khosravi, F, Upadhyay, M, Kumar, A, Shahsavani, M. B, Akbarian, M, Najafi, H, Tamaddon A.M, Yousefi, R, A novel method for the chaperone aided and efficient production of human proinsulin in the prokaryotic system. Journal of Biotechnology, 2022,  DOI: 10.1016/j.jbiotec.2022.01.002
  • Khoshaman, K, Ghahramani, M, Shahsavani, M. B, Moosavi-Movahedi, A. A, Kurganov, B. I,  Yousefi, R, Myopathy-associated G154S mutation causes important changes in the conformational stability, amyloidogenic properties, and chaperone-like activity of human αB-crystallin. Biophysical Chemistry, 2022, 282, 106744, https://doi.org/10.1016/j.bpc.2021.106744.
  •   Moghadam, S. S, Ghahramani, M, Khoshaman, K, Oryan, A, Moosavi-Movahedi, A. A, Kurganov, B. I,  Yousefi, R, Relationship between the structure and chaperone activity of human αA-Crystallin after its modification with diabetes-associated oxidative agents and protective role of antioxidant compounds. Biochemistry (Moscow), 2022 Feb;87(2):91-105. doi: 10.1134/S000629792202002X.
  • Yousefi, R, Crystallins as Important Pathogenic Targets for Accumulation of Structural Damages Resulting in Protein Aggregation and Cataract Development: Introduction to This Special Issue of Biochemistry (Moscow). Biochemistry (Moscow), 2022, 87(2), 87-90.  DOI: 10.1134/S0006297922020018
  • Yousefi, A, Ahrari, S, Panahi, F, Ghasemi, Y, Yousefi, R, Binding analysis of the curcumin-based synthetic alpha-glucosidase inhibitors to beta-lactoglobulin as potential vehicle carrier for antidiabetic drugs. Journal of the Iranian Chemical Society, 2022, 19 (2), 489-503. DOI:10.1007/s13738-021-02323-8
  • Barati, A, Masoudi, R, Yousefi, R, Monsefi, M, & Mirshafiey, A, Tau and amyloid beta differentially affect the innate immune genes expression in Drosophila models of Alzheimer's disease and β-D Mannuronic acid (M2000) modulates the dysregulation. Gene, 2022, 808, 145972.  DOI: 10.1016/j.gene.2021.145972
  • Hafizi, M, Chebotareva, N. A, Ghahramani, M, Moosavi-Movahedi, F, Khaleghinejad, S. H, Kurganov, B. I, Yousefi, R, Structural and functional studies of D109A human αB-crystallin contributing to the development of cataract and cardiomyopathy diseases. PLOS ONE, 2021, 16(11), e0260306, https://doi.org/10.1371/journal.pone.0260306
  • Nasiri, P, Ghahramani, M, Tavaf, Z, Niazi, A, Moosavi-Movahedi, A. A, Kurganov, B. I, Yousefi, R, The biochemical association between R157H mutation in human αB-crystallin and development of cardiomyopathy: Structural and functional analyses of the mutant protein. 2021, Biochimie, 190, 36-49. DOI: 10.1016/j.biochi.2021.06.019
  • Mirzapour-Kouhdasht A, Moosavi-Nasab M, Yousefi R, Eun J.B, Bio/multi-functional peptides derived from fish gelatin hydrolysates: Technological and functional properties, Biocatalysis and Agricultural Biotechnology, 2021, 36, 102152. DOI: 10.1038/s41598-021-86808-9
  •  Ashrafi A,  Alavianmehr M.M,  Mohammad-Aghaie D, Yousefi R, Golbon Haghighi M, Moosavi-Movahedi A.A, Soltani Rad N, Insights into Platinum Complex-HSA Bindings: Spectral and Molecular Dynamics Simulation Studies, Physical Chemistry Research, 2021, 9 (3): 427-446.  10.22036/PCR.2021.252500.1845
  • Farahavar G,  Abolmaali S.S, Nejatollahi F, Safaie A, Javanmardi S, Khajeh Zadeh H, Yousefi R, Nadgaran H,  Mohammadi-Samani S, Tamaddon A.M, Ahadian S,  Single-chain antibody-decorated Au nanocages@ liposomal layer nanoprobes for targeted SERS imaging and remote-controlled photothermal therapy of melanoma cancer cells, Materials Science and Engineering: C, 2021, 124, 112086. https://doi.org/10.1016/j.msec.2021.112086
  • Akbarian M., Yousefi R., Farjadian F., Uversky V., Insulin fibrillation: toward strategies for attenuating the process, Chemical Communications, 2020, DOI: 10.1039/d0cc05171c.
  •  Akbarian M., Kianpour M., Yousefi R., Moosavi-Movahedi AA, Characterization of insulin cross-seeding: the underlying mechanism reveals seeding and denaturant-induced insulin fibrillation proceeds through structurally similar intermediates, RSC Adv., 2020, 10, 29885–29899, 2020 DOI: 10.1039/d0ra05414c
  • Tavaf  Z, Khajeh Dangolani S,  Yousefi R, Panahi F, Shahsavani M.B, Khalafi-Nezhad A, Synthesis of new curcumin derivatives as influential antidiabetic α-glucosidase and α-amylase inhibitors with anti-oxidant activity, Carbohydrate Research, 2020, 494:108069. DOI:10 Jun 2020, 494:108069.
  • Yousefi R, Moosavi-Movahedi A.A, Achilles' heel of the killer virus: the highly important molecular targets for hitting SARS-CoV-2 that causes COVID-19, Journal of the Iranian Chemical Society, 2020, 17:1257–1258,  Editorial, published: 24 April 2020.
  • Ghahramani M, Yousefi R, Khoshaman K, Sasan Moghadam S, and Kurganov B, Analysis of the data on titration of native and peroxynitrite modified αA- and αB-crystallins by Cu2+-ions, Data Brief,  2020, doi: 10.1016/j.dib.2020.105492.
  • Ghahramani M, Yousefi R, Niazi A, Kurganov B, The congenital cataract-causing mutations P20R and A171T are associated with important changes in the amyloidogenic feature, structure and chaperone-like activity of human αB-crystallin, Biopolymers, 2020 Feb 28:e23350. doi: 10.1002/bip.23350.
  • Ghahramani M, Yousefi R, Krivandin A, Muranov K, Kurganov B, Moosavi-Movahedi AA,  Kinetic data analysis of chaperone-like activity of Wt, R69C and D109H αB-crystallins, Data Brief, 2020, 28:104922. doi: 10.1016/j.dib.2019.104922. eCollection 2020 Feb.
  •  Ghahramani M, Yousefi R, Krivandin A, Muranov K, Kurganov B, Moosavi-Movahedi AA, Structural and functional characterization of D109H and R69C mutant versions of human αB-crystallin: The biochemical pathomechanism underlying cataract and myopathy development, Int J Biol Macromol., 2020, 146:1142-1160. doi: 10.1016/j.ijbiomac.2019.09.239.
  •  Alavianmehr MM, Ashrafi A, Yousefi R, Haghighi MG,  Abolmaali SS,  Rad MS, Moosavi-Movahedi AA, Anticancer activity assessment and DNA binding properties of two binuclear platinum (II) complexes using spectroscopic and molecular simulation approaches, Anticancer Agents Med Chem. 2020,  doi: 10.2174/1871520620666200705221325. Online ahead of print.
  •  Akbarian M, Yousefi R, Moosavi-Movahedi AA, Ahmad A, Uversky VN, Modulating Insulin Fibrillation Using Engineered B-Chains with Mutated C-Termini, Biophys J. 2019 Sep 23. pii: S0006-3495(19)30797-0. doi: 10.1016/j.bpj.2019.09.022.
  • Kianpour M, Yousefi R, Carnosine prevents different structural damages induced by methylglyoxal in lens, Cell Biochem Biophys., 2019 Dec;77(4):343-355. doi: 10.1007/s12013-019-00884-3.
  • Mehraban MH, Mansourian M, Ahrari S, HajiEbrahimi A, Odooli S, Motovali-Bashi M, Yousefi R, Ghasemi Y (2019) Maltase-glucoamylase inhibition potency and cytotoxicity of pyrimidine-fused compounds: An in silico and in vitro approach, Comput Biol Chem, 82:25-36. doi: 10.1016/j.compbiolchem. 
  • Ashoo P, Yousefi R, Nabavizadeh SM, Aseman MD, Paziresh S, Ghasemi A, Saboury AA (2019) Three Pt-Pt complexes with donor-acceptor feature: anticancer activity, DNA binding studies and molecular docking simulation, Anticancer Agents Med Chem, 2019;19(14):1762-1774. doi: 10.2174/1871520619666190702114211.
  •  Amirahmadi M, Salesi M, Yousefi R, Daryanosh F, Nemati J, Kurganov BI, The impact of concurrent training and antioxidant supplementation on the factors associated with the ocular lens opacity in diabetic rats, Arch Physiol Biochem. 2019 Oct 1:1-15. doi: 10.1080/13813455.2019.1668019. [Epub ahead of print].
  • Akbarian M, Yousefi R, Human alpha B-crystallin as fusion protein and molecular chaperone increases the expression and folding efficiency of recombinant insulin, PLoS One, 2018 Oct 19;13(10):e0206169. doi: 10.1371/journal.pone.0206169. eCollection 2018.
  • Anbaraki A, Ghahramany M, Muranov K, Kurganov B, Yousefi R, Structural and functional alteration of human alpha A-crystallin after exposure to full spectrum solar radiation and preventive role of lens antioxidants, Int J Biol Macromol. 2018 Oct 15;118(Pt A):1120-1130. doi: 10.1016/j.ijbiomac.2018.06.136. Epub 2018 Jun 30.
  • Akbarian M, Ghasemi Y, Uversky V, Yousefi R, Chemical modifications of insulin: Finding a compromise between stability and pharmaceutical performance, Int J Pharm. 2018 Aug 25;547(1-2):450-468. doi: 10.1016/j.ijpharm.2018.06.023. Epub 2018 Jun 8.
  • Khajeh dangolani S, Panahi F, Tavaf Z, Nourisefat M, Yousefi R, Khalafi-Nezhad A, Synthesis and Antioxidant Activity Evaluation of Some Novel Aminocarbonitrile Derivatives Incorporating Carbohydrate Moieties. ACS Omega.  2018 Aug 31;3(8):10341-10350. doi: 10.1021/acsomega.8b01124. eCollection 2018 Aug 31. Erratum in: ACS Omega. 2019 Mar 26;4(3):5862.
  • Moghadam SS, Oryan A, Kurganov BI, Tamaddon AM, Alavianehr MM, Moosavi-Movahedi AA, Yousefi R, The structural damages of lens crystallins induced by peroxynitrite and methylglyoxal, two causative players in diabetic complications and preventive role of lens antioxidant components, Int J Biol Macromol. 2017 Oct;103:74-88. doi: 10.1016/j.ijbiomac.2017.04.090. Epub 2017 May 1.
  • Khoshaman K, Yousefi R, Niazi A, Oryan A, Moosavi-Movahedi AA, Kurganov BI, Importance of the positively charged residue at position 54 to the chaperoning function, conformational stability and amyloidogenic nature of human αA-crystallin, J Biochem. 2018 Mar 1;163(3):187-199. doi: 10.1093/jb/mvx071.
  • Khoshaman K, Yousefi R, Moosavi-Movahedi AA, Protective role of antioxidant compounds against peroxynitrite-mediated modification of R54C mutant αA-crystallin, Arch Biochem Biophys. 2017 Sep 1;629:43-53. doi: 10.1016/j.abb.2017.07.007. Epub 2017 Jul 16.
  • Sadeghian T, Tavaf Z, Oryan A, Shokouhi R, Pourpak Z, Moosavi-Movahedi AA, Yousefi R, Structure, chaperone-like activity and allergenicity profile of bovine caseins upon peroxynitrite modification: New evidences underlying mastitis pathomechanisms, Int J Biol Macromol. 2018 Jan;106:1258-1269. doi: 10.1016/j.ijbiomac.2017.08.120. Epub 2017 Aug 26.
  • Yousefi R, Saba S, Ghahramani M, A comparative study of the impact of calcium ion on structure, aggregation and chaperone function of human αA-crystallin and its cataract-causing R12C mutant, Protein Pept Lett. 2017;24(11):1048-1058. doi: 10.2174/0929866524666170807125658.
  •   Najjar FM, Ghadari R, Yousefi R, Safari N, Sheikhhasani V, Sheibani N, Moosavi-Movahedi AA, Studies to reveal the nature of interactions between catalase and curcumin using computational methods and optical techniques, Int J Biol Macromol. 2017 Feb;95:550-556. doi: 10.1016/j.ijbiomac.2016.11.050. Epub 2016 Nov 16.
  •    Khoshaman K, Masoudi R, Hemmateenejad B, Yousefi R, The structural alteration and aggregation propensity of glycated lens crystallins in the presence of calcium: Importance of lens calcium homeostasis in development of diabetic cataracts, Spectrochim Acta A Mol Biomol Spectrosc. 2017 Jan 5;170:174-83. doi: 10.1016/j.saa.2016.07.017. Epub 2016 Jul 9.
  •    Asadi Z, Mosallaei H, Sedaghat M, Yousefi R, Competitive binding affinity of two lanthanum (III) macrocycle complexes toward DNA and bovine serum albumin in water, J Iran Chem Soc.14(11).2017. DOI:  10.1007/s13738-017-1172-3
  •     Yousefi R, Ferdowsi L, Tavaf Z, Sadeghian T, Tamaddon AM, Moghtaderi M, Pourpak Z, Evaluation of Structure, Chaperone-Like Activity and Allergenicity of Reduced Glycated Adduct of Bovine β-casein, Protein Pept Lett. 2017;24(1):46-55. doi: 10.2174/0929866524666161121144025.
  •    Khoshaman K, Yousefi R, Tamaddon AM, Abolmaali SS, Oryan A, Moosavi-Movahedi AA, Kurganov BI, The impact of different mutations at Arg54 on structure, chaperone-like activity and oligomerization state of human αA-crystallin: The pathomechanism underlying congenital cataract-causing mutations R54L, R54P and R54C, Biochim Biophys Acta. 2017, pii: S1570-9639 (17) 30018-3. doi: 10.1016/j.bbapap.2017.02.003.
  •      Zafaranchi ZM S, Khoshaman K, Masoudi R, Hemmateenejad B, Yousefi R, The structural alteration and aggregation propensity of glycated lens crystallins in the presence of calcium: Importance of lens calcium homeostasis in development of diabetic cataracts, Spectrochim Acta A Mol Biomol Spectrosc. 2017 Jan 5;170:174-83. doi: 10.1016/j.saa.2016.07.017. Epub 2016 Jul 9.
  •  Shahsavai MB, Ahmadi S, Dadkhah-Aseman M, Nabavizadeh SM, Alavianmer MM, Yousefi R, Comparative study on the interaction of two binuclear Pt (II) complexes with human serum albumin: Spectroscopic and docking simulation assessments, J Photochem Photobiol B. 2016 Nov;164:323-334. doi: 10.1016/j.jphotobiol.2016.09.035. Epub 2016 Sep 30.
  •   Moafian Z, Khoshaman K, Oryan K, Kurganov BI, Yousefi R, Protective effects of acetylation on the pathological reactions of the lens crystallins with homocysteine thiolactone. PLoS One. 2016 Oct 5;11(10):e0164139. doi: 10.1371/journal.pone.0164139. eCollection 2016.
  •    Anbaraki A, Khoshaman K, Ghasemi Y, Yousefi R,  Antioxidant defense mechanism against riboflavin-mediated sunlight damaging of lens crystallins, Int J Biol Macromol. 2016 Oct;91:895-904. doi: 10.1016/j.ijbiomac.2016.06.047. Epub 2016 Jun 15.
  •     Mehraban MH, Odooli S, Yousefi R, Roghanian R, Motovali-Bashi M, Moosavi-Movahedi AA, Ghasemi Y (2016) The interaction of beta-lactoglobulin with ciprofloxacin and kanamycin; a spectroscopic and molecular modeling approach, J Biomol Struct Dyn. 2017 Jul;35(9):1968-1978. doi: 10.1080/07391102.2016.1203819. Epub 2016 Sep 19.
  •    Javadi S, Yousefi R, Hosseinkhani S, Tamaddon A, Uversky V, Protective effects of carnosine on dehydroascorbate-induced structural alteration and opacity of lens crystallins: Important implications of carnosine pleiotropic functions to combat cataractogenesis J Biomol Struct Dyn. 2017 Jun;35(8):1766-1784. doi: 10.1080/07391102.2016.1194230. Epub 2016 Aug 19.
  •   Shahsavani MB, Ahmadi SH, Dadkhah Aseman M, Nabavizadeh SM, Rashidi M, Asadi Z, Erfani N, Ghasemi A, Saboury AA, Niazi A, Bahaoddini A, Yousefi R, Anticancer activity assessment of two novel binuclear platinum (II) complexes, J Photochem Photobiol B. 2016 Aug;161:345-54. doi: 10.1016/j.jphotobiol.2016.05.025. Epub 2016 Jun 2.
  •    Ghahramani M, Yousefi R, Khoshaman K, Moghadam SS, Kurganov BI, Evaluation of structure, chaperone-like activity and protective ability of peroxynitrite modified human α-Crystallin subunits against copper-mediated ascorbic acid oxidation, Int J Biol Macromol. 2016 Jun;87:208-21. doi: 10.1016/j.ijbiomac.2016.02.040. Epub 2016 Feb 16.
  •   Rastegari B, Karbalaei-Heidari H, Yousefi R, Zeinali S, Nabavizadeh S, Interaction of prodigiosin with HSA and β-Lg: Spectroscopic and molecular docking studies, Bioorg Med Chem. 2016 Apr 1;24(7):1504-12. doi: 10.1016/j.bmc.2016.02.020. Epub 2016 Feb 16.
  •    Bahmari F, Panahi F, Daneshgar F, Yousefi R, Shahsavani M, Khalafinezhad A (2016) Synthesis of new α-aminophosphonate derivatives incorporating benzimidazole, theophylline and adenine nucleobases using L-cysteine functionalized magnetic nanoparticles (LCMNP) as magnetic reusable catalyst: Evaluation of their anticancer activities, RSC Advances, 6:5915-5924. 2016. DOI:10.1039/C5RA21419J.
  •    Ragerdi Kashani M, Yousefi R, Akbarian M, Alavianmehr M, Ghasemi Y (2016) Structure, chaperone activity and aggregation of wild-type and R12C mutant alphaB-crystallins in the presence of thermal stress and calcium ion; implications for the role of calcium in cataract pathogenesis, Biochemistry (Mosc). 2016 Feb;81(2):122-34. doi: 10.1134/S0006297916020061.
  •  Hajari S, Masoudi R, Javadi S, Hemmateenejad B, Yousefi R, The structural alteration and aggregation of bovine lens gamma-crystallin by homocysteinylation; the pathomechanism underlying cataract development during hyperhomocysteinimia, Protein Pept Lett. 2016;23(1):78-86. DOI: 10.2174/0929866523666151106123944.  
  •  Yousefi R, Jamshidi M, Shahsavani M, Nabavizadeh S M, Golbon Haghighi M, Rashidi M, Taheri-Kafrani A, Niazi A, Keshavarz F, Alavinamehr MM, Study on the interaction of three structurally related cationic Pt (II) complexes with human serum albumin: importance of binding affinity and denaturing properties, J Iran Chem Soc, 13(4):617-630.  2015. DOI:10.1007/s13738-015-0773-y.
  •    Yousefi R, Javadi S, Amirghofran S, Oryan A, Moosavi-Movahedi A, Assessment of structure, stability and aggregation of soluble lens proteins and alpha-crystallin upon non-enzymatic glycation: The pathomechanisms underlying cataract development in diabetic patients, Int J Biol Macromol. 2016 Jan;82:328-38. doi: 10.1016/j.ijbiomac.2015.10.036. Epub 2015 Oct 23.
  •     Yousefi R, Taheri B, Alavi P, Shahsavani M, Asadi Z, Ghahramany M, Niazi A, Alavian mehr M, Moosavi-Movahedi A, Aspirin-mediated acetylation induces structural alteration and aggregation of bovine pancreatic insulin, J Biomol Struct Dyn. 2016;34(2):362-75. doi: 10.1080/07391102.2015.1039584. Epub 2015 Jun 23.
  • Yousefi R, Taheri-Kafrani A, Nabavizadeh S, Pouryasin Z, Shahsavani M, Khoshaman K, Rashidi M (2015) The binding assessment with human serum albumin of novel six-coordinate Pt(IV) complexes, containing bidentate nitrogen donor/methyl ligands, Mol Biol Res Commun. 2015 Dec;4(4):167-179.
  • Khoshaman K, Yousefi R, Saso L, Moosavi-Movahedi A, The impact of hydrogen peroxide on structure, stability and functional properties of human R12C mutant< alpha>A-Crystallin: the imperative insights into pathomechanism of the associated congenital cataract incidence, Free Radic Biol Med. 2015 Dec;89:819-30. doi:  10.1016/j.freeradbiomed.2015.09.013. Epub 2015 Oct 12.
  •     Rezanejad Bardeji H, Karbalaei-Heidari H, Rezaei S, Yousefi R, Microsciadin, a New Milk-Clotting Cysteine Protease from an Endemic Species, Euphorbia microsciadia, Biomacromolecular Journal, 2015; 1:93-103.
  •     Toobaei Z, Yousefi R, Panahi F, Shahidpour S, Nourisefat M, Doroodmand M, Khalafinezhad A, Synthesis of novel poly-hydroxyl functionalized acridine derivatives as inhibitors of alpha-Glucosidase and alpha-Amylase, Carbohydr Res. 2015 Jun 26;411:22-32. doi: 10.1016/j.carres.2015.04.005. Epub 2015 Apr 18.
  •   Yousefi A, Yousefi R, Panahi F, Sarikhani-Khorrami F, Bahaoddini A, Zolghadr A, Khalafinezhad A, Novel curcumin-based pyrano [2, 3-d] pyrimidine anti-oxidant inhibitors for alpha-amylase and alpha-glucosidase; implications for their pleiotropic effects against diabetes complications, Int J Biol Macromol. 2015;78:46-55. doi: 10.1016/j.ijbiomac.2015.03.060. Epub 2015 Apr 3.
  •   Shahidpour S, Panahi F, Yousefi R, Nourisefat M, Nabipour M, Khalafinezhad A, Design and synthesis of new antidiabetic alpha-glucosidase and alpha-amylase inhibitors based on pyrimidine-fused heterocycles, Med Chem Res. 2015; 24:3086-3096. DOI:10.1007/s00044-015-1356-2.
  •   Ghahramany M, Yousefi R, Khoshaman K, Alavian mehr M, The impact of calcium ion on structure and aggregation propensity of peroxynitrite-modified lens crystallins; new insights into the pathogenesis of cataract disorders, Colloids Surf B Biointerfaces. 2015 Jan 1;125:170-80. doi: 10.1016/j.colsurfb.2014.11.002.
  •    Yousefi R, Mohammadi R, Taheri-Kafrani A, Sahahsavani M, Dadkhah aseman M, Nabavizadeh S, Rashidi M, Poursasan N, Moosavi Movahedi A, Study of the interaction between two newly synthesized cyclometallated platinum (II) complexes and human serum albumin: Spectroscopic characterization and docking simulation, Journal of Luminescenceis, 2015; 159:139-146. DOI: 10.1016/j.jlumin.2014.10.055.
  •   Mohammadi R, Yousefi R, Dadkhah aseman M, Nabavizadeh S, Rashidi M, DNA binding and anticancer activity of novel cyclometalated platinum (II) complexes, Anticancer Agents Med Chem. 2015;15:107-114. DOI:  10.2174/1871520614666141203143543.
  •   Khalafinezhad A, Panahi F, Yousefi R, Sarrafi S, Gholamalipour Y, Magnetic nanoparticles-supported tungstosilicic acid: as an efficient magnetically separable solid acid for the synthesis of benzoazoles in water, J Iran Chem Soc. 2014; 11:1311-1319. DOI:10.1007/s13738-013-0400-8.
  •   Khalafinezhad A, Panahi F, Yousefi R, Gholamalipour Y, Sarrafi S, An efficient procedure for the synthesis of phenacyl and benzyl azolium salts using fluorous alcohols, J Iran Chem Soc. 2013; 11:1189-1196. DOI:10.1007/s13738-013-0387-1.
  •   Yousefi R, Alavianmehr M, Mokhtari F, Keshavarz F, Nabavizadeh S, Rashidi M, Moosavi-Movahedi A (2014) Spectroscopic and Molecular Dynamics Studies on Binding of Dimethyplatinum(II) Complex Drug-Based to Human Serum Albumin, Bulletin of the Chemical Society of Japan 87(10):1094-1100. DOI: 10.1246/bcsj.20130319.
  •  Alavianmehr M, Yousefi R, Keshavarz F, Mohammad Aghaei D, Probing the Binding of ThioTEPA to Human Serum Albumin, Using Spectroscopic and Molecular Simulation Approaches, Canadian Journal of Chemistry.2014; 92:1066-1073. DOI: 10.1139/cjc-2013-0571.
  •   Asadi Z, Asadi M, Dehghani firuzabadi F, Yousefi R, Jamshidi M, Synthesis, characterization, anticancer activity,thermal and electrochemical studies of some novel uranyl Schiff base complexes,  J Iran Chem Soc.2014; 11:423-429. DOI: 10.1007/s13738-013-0314-5
  •  Jamshidi M, Yousefi R, Nabavizadeh S, Rashidi M, Golbon Haghighi M, Niazi A, Moosvi movahedi A, Anticancer activity and DNA-binding properties of novel cationic Pt (II) complexes, Int J Biol Macromol. 2014 May;66:86-96. doi: 10.1016/j.ijbiomac.2014.01.057. Epub 2014 Feb 12.
  •  Pouryasin Z, Yousefi R, Nabavizadeh S, Rashidi M, Hamidizadeh P, Alavian mehr M, Moosvi movahedi A (2014) Anticancer and DNA Binding Activities of Pt(IV) Platinum Complexes; Importance of Leaving Group Departure Rate, Appl Biochem Biotechnol. 2014 Mar;172(5):2604-17. doi: 10.1007/s12010-013-0700-6. Epub 2014 Jan 12.
  •  Ariaeenejad S, Moosavi-Movahedi A, Kavousi K, Dayer M, Hong J, Yousefi R, Sheibani N, Habibi-Rezaei M (2014) The species and heme pocket properties of Sturgeon hemoglobins upon interaction with n-dodecyl trimethylammonium bromide, Protein Pept Lett. 2014;21(2):171-8. DOI: 10.2174/09298665113209990092.
  •  Aghevlian S, Yousefi R, Faghihi R, Abbaspour A, Niazi A, Jaberipour M, Hosseini A (2012) The improvement of anti-proliferation activity against breast cancer cell line of thioguanine by gold nanoparticles, Med Chem Res., 2013; 22:303-311. DOI:10.1007/s00044-012-0030-1.
  •  Taghavi F, Moosavi-Movahedi A, Bohloolo , Alijanvand H, Salami M, Maghami P, Saboury A, Farhadi M, Yousefi R, Habibi-Rezaei M, Sheibani M, Potassium sorbate as an AGE activator for human serum albumin in the presence and absence of glucose, Int J Biol Macromol. 2013 Nov;62:146-54. doi: 10.1016/j.ijbiomac.2013.08.045.
  •  Keshavarz F, Alavianmehr M, Yousefi R, Molecular interaction of benzalkonium ibuprofenate and its discrete ingredients with human serum albumin, Phy Chem Res. 2013; 1:111-116, 10.22036/PCR.2013.3039.
  •  Panahi F, Yousefi R, Mehraban M, Khalafinezhad A, Synthesis of new pyrimidine-fused derivatives as potent and selective antidiabetic alpha-glucosidase inhibitors, Carbohydr Res. 2013 Oct 18;380:81-91. doi: 10.1016/j.carres.2013.07.008.
  •  Mehraban M, Yousefi R, Taheri-Kafrani A, Panahi F, Khalafinezhad A, Binding study of novel anti-diabetic pyrimidine fused heterocycles to beta-lactoglobulin as a carrier protein, Colloids Surf B Biointerfaces. 2013 Dec 1;112:374-9. doi: 10.1016/j.colsurfb.2013.08.013.
  •  Alavi P, Yousefi R, Amirghofran S, Karbalaei-Heidari H, Moosavi-Movahedi A, Structural analysis and aggregation propensity of reduced and non-reduced glycated insulin adducts, Applied Biochemistry and Biotechnology, Appl Biochem Biotechnol. 2013 Jun;170(3):623-38. doi: 10.1007/s12010-013-0207-1.
  •  Yousefi R, Khazaei S, Moosavi-Movahedi A, Effect of homocysteinylation on structure, chaperone activity and fibrillation propensity of lens alpha-crystallin, Protein Pept Lett. 2013 Aug;20(8):932-41. DOI: 10.2174/0929866511320080011.
  •  Asadi M, Savaripour A, Asadi Z, Ghatee M, Moosavi Baygi F, Yousefi R, Jamshidi M, Synthesis and characterization of some new Schiff base complexes of group 13 elements, ab initio studies, cytotoxicity and reaction with hydrogen peroxide, Spectrochim Acta A Mol Biomol Spectrosc. 2013 Jan 15;101:394-9. doi: 10.1016/j.saa.2012.09.007. 
  •   Keshavarz F, Alavian mehr M, Yousefi R, Molecular dynamics simulation and docking studies on the binding properties of several anticancer drugs to human serum albumin, Mol Biol Re Comm., 2012; 2:65-73.
  •   Riazi G, Karima O, Khodadadi S, Yousefi R, Mahnam K, Mokhtari F, Cheraghi T, Hoveizi E, Moosavi-Movahedi A, An in vitro study of the role of beta-boswellic acid in the microtubule assembly dynamics, FEBS Lett. 2012 Nov 30;586(23):4132-8. doi: 10.1016/j.febslet.2012.10.007.
  •     Khazaei S, Yousefi R, Alavian mehr M, Aggregation and fibrillation of eye lens crystallins by homocysteinylation; implication in the eye pathological disorders, Protein J. 2012 Dec;31(8):717-27. doi: 10.1007/s10930-012-9451-4.
  •   Yousefi R, Alavian mehr M, Mokhtari F, Panahi F, Mehraban M, Khalafinezhad A, Pyrimidine-fused heterocycle derivatives as a novel class of inhibitors for alpha-glucosidase, J Enzyme Inhib Med Chem. 2013 Dec;28(6):1228-35. doi: 10.3109/14756366.2012.727812.
  •   Sharifizadeh A, Saboury A, Moosavi Movahedi A, Maryam S, Yousefi R, A new aspect to chaperone-like activity of bovine beta-casein by protein-protein interactions study, Int J Biol Macromol. 2012 Dec;51(5):901-7. doi: 10.1016/j.ijbiomac.2012.08.002.
  •   Yousefi R, Aghevlian S, Mokhtari F, Samouei H, Rashidi M, Nabavizadeh S, Tavaf langeroudi Z, Pouryasin Z, Niazi A, Faghihi R, Papari M, The anticancer activity and HSA binding properties of thThe anticancer activity and HSA binding properties of the structurally related platinum (II) complexes, Appl Biochem Biotechnol. 2012 Jun;167(4):861-72. doi: 10.1007/s12010-012-9733-5.
  •   Asadi M, Barzegar S, Asadi Z, Yousefi R, Sa'adi B, Khalili Hezarjaribi H, Synthesis, characterization, and thermodynamic studies of the interaction of some new water-soluble Schiff-base complexes with bovine serum albumin, J Cord Chem. 2012; 65:722-39. DOI:  10.1080/00958972.2012.661419.
  •   Yousefi R, Jalili S, Alavi P, Moosavi Movahedi A, The enhancing effect of homocysteine thiolactone on insulin fibrillation and cytotoxicity of insulin fibril, Int J Biol Macromol. 2012 Oct;51(3):291-8. doi: 10.1016/j.ijbiomac.2012.05.021.
  •    Khalili Hezarjaribi H, Yousefi R, Moosavi-Movahedi A, Effect of temperature and ionic strength on structure and chaperone activity of glycated and non-glycated alpha-crystallins, Protein Pept Lett. 2012 Apr;19(4):450-7. DOI: 10.2174/092986612799789396.
  •    Yousefi R, Khalafinezhad A, Soltani Rad M, Behrouz, Panahi F, Esmaili M, Ghaffari S, Niazi A, Moosavi-Movahedi A, Structure–cytotoxicity relationship of a novel series of miconazole-like compounds, Med Chem Res., 2011; 21:1921-1928. DOI:10.1007/s00044-011-9716-z.
  •    Salami M, Moosavi-Movahedi A, Moosavi-Movahedi F, Ehsani M, Yousefi R, Farhadi M, Niasari-Naslaji A, Saboury A, Chobert J, Haertle T, Biological activity of camel milk casein following enzymatic digestion, J Dairy Res. 2011 Nov;78(4):471-8. doi: 10.1017/S0022029911000628.
  •    Yousefi R, Jalili S, The synergistic chaperoning operation in a Bi-chaperone system consisting of alpha-crystallin and beta-casein: bovine pancreatic insulin as the target protein, Colloids Surf B Biointerfaces. 2011 Nov 1;88(1):497-504. doi: 10.1016/j.colsurfb.2011.07.040.
  •    Esmaeili M, Ghaffari S, Moosavi-Movahedi Z, Atri M, Sharifzadeh A, Farhadi M, Yousefi R, Chobert J, Haertle T, Moosavi-Movahedi A, Beta casein-micelle as a nano vehicle for solubility enhancement of curcumin; food industry application, LWT-Food Science and Technology,2011; 44:2166-2172. DOI: 10.1016/j.lwt.2011.05.023.
  •    Stroylova Y, Zimny J, Yousefi R, Chobert J, Jakubowski H, Muronetz V, Haertle T, Aggregation and structural changes of alpha-S1-, beta- and kappa-caseins induced by homocysteinylation, Biochim Biophys Acta. 2011 Oct;1814(10):1234-45. doi: 10.1016/j.bbapap.2011.05.017.
  •    Miroliaei M, Shirazi M, Yousefi R, A Comparative study on the chaperone-like activity of camel and bovine beta-caseins, Journal of Paramedical Sciences, 2011; 2:13-19. DOI: https://doi.org/10.22037/jps.v2i1.2142.
  •    Jalili S, Yousefi R, Moosavi-Movahedi A, Papari M, Effect of Homocysteine Thiolactone on Structure and Aggregation Propensity of Bovine Pancreatic Insulin, Protein J. 2011 Jun;30(5):299-307. doi: 10.1007/s10930-011-9333-1.
  •    Atri M, Saboury A, Yousefi R, Delgalarando M, Chobert J, Haertle T, Moosavi-Movahedi A, Comparative study on heat stability of camel and bovine apo and holo alpha-lactalbumin, J Dairy Res. 2010 Feb;77(1):43-9. doi: 10.1017/S0022029909990367.
  •    Salami M, Moosavi-Movahedi A, Ehsani M, Yousefi R, Haertle T, Chobert J, Razavi S, Henrich R, Balalaei S, Ebadi S, Poutakdoost S, Niasari-Naslaji A, Improvement of the Antimicrobial and Antioxidant Activities of Camel and Bovine Whey Proteins by Limited Proteolysis, J Agric Food Chem. 2010 Mar 24;58(6):3297-302. doi: 10.1021/jf9033283.
  •    Karima O, Riazi G, Yousefi R, Moosavi-Movahedi A, The enhancement effect of beta-boswellic acid on hippocampal neurites outgrowth and branching (an in vitro study), Neurol Sci. 2010 Jun;31(3):315-20. doi: 10.1007/s10072-010-0220-x.
  •   Gaudin J, Le Parc A, Castrec B, Ropers M, Choiset Y, Shchutskaya J, Yousefi R, Muronetz V, Zuev Y, Chobert J, Heartle T, Engineering of caseins and modulation of their structures and interactions, Biotechnol Adv. 2009 Nov-Dec;27(6):1124-1131. doi: 10.1016/j.biotechadv.2009.05.011.
  •    Badraghi J, Moosavi-Movahedi A, Saboury A, Yousefi R, Sharifizadeh A, Hong J, Haertle T, Niasari-Naslaji A, Sheibani N, Dual behavior of sodium dodecyl sulfate as enhancer or suppressor of insulin, Int J Biol Macromol. 2009 Dec 1;45(5):511-7. doi: 10.1016/j.ijbiomac.2009.08.008. 
  •    Salami M, Yousefi R, Ehsani M, Razavi S, Chobert J, Haertle T, Saboury A, Atri M, Niasari-Naslaji A, Ahmad F, Moosavi-Movahedi A, Enzymatic digestion and antioxidant activity of the native and molten globule, International Dairy Journal, 2009; 19:518-523. https://doi.org/10.1016/j.idairyj.2009.02.007.
  •    Badraghi J, Yousefi R, Saboury A, Sharifizadeh A, Haertle T, Ahmad F, Moosavi-Movahedi A, Effect of salts and sodium dodecyl sulfate on chaperone activity of camel [α]S1-CN, Colloids Surf B Biointerfaces. 2009 Jul 1;71(2):300-5. doi: 10.1016/j.colsurfb.2009.03.008.
  •    Yousefi R, Shchutskaya Y, Zimny J, Gaudin J, Moosavi-Movahedi A, Muronetz V, Zuev Y, Chobert J, Haertle T, Chaperone-Like Activities of Different Molecular Forms of beta-casein, Biopolymers. 2009 Aug;91(8):623-32. doi: 10.1002/bip.21190.
  •    Yousefi R, Gaudin J, Chobert J, Pourpak Z, Moeen M, Moosavi-Movahedi A, Haertle T, Micellisation and immunoreactivities of dimeric beta-caseins, Biochim Biophys Acta. 2009 Dec;1794(12):1775-83. doi: 10.1016/j.bbapap.2009.08.015.
  •   Barzegar A, Yousefi R, Sharifzadeh A, Dalgalarrondo M, Chobert J, Ganjali M, Norozi P, Ehsani M, Niasari-Naslaji A, Saboury A, Haertle T, Moosavi-Movahedi A, Chaperone activities of bovine and camel beta-caseins: Importance of their surface hydrophobicity in protection against alcohol dehydrogenase aggregation, Int J Biol Macromol. 2008 May 1;42(4):392-9. doi: 10.1016/j.ijbiomac.2008.01.008.
  •     Zali H, Rezaei-Tavirani M, Kariminia A, Yousefi R, Shokrgozar M, Evaluation of growth inhibitory and apoptosis inducing activity of human calprotectin on the human gastric cell line, Iran Biomed J. 2008 Jan;12(1):7-14.
  •   Hassanisadi M, Barzegar A, Yousefi R, Delgalarando M, Chobert J, Haertle T, Saboury A, Moosavi-Movahedi A, Chemometric study of the aggregation of alcohol dehydrogenase and its suppression by beta-caseins: a mechanistic perspective, Anal Chim Acta. 2008 Apr 14;613(1):40-7. doi: 10.1016/j.aca.2008.02.036.
  •   Amani M, Yousefi R, Moosavi-Movahedi A, Pintus, Mura A, Floris , Kurganov B, Saboury A, Structural changes and aggregation process of cu/containing amine oxidase in the presence of 2,2,2'-trifluoroethanol, Protein Pept Lett. 2008;15(5):521-7. DOI: 10.2174/092986608784567636.
  •    Salami M, Yousefi R, Ehsani M, Dalgalarrondo M, Chobert J, Haertle T, Razavi S, Saboury A, Niasari-Naslaji A, Moosavi-Movahedi A, Kinetic characterization of hydrolysis of camel and bovine, Biochem Biophys Res Commun. 2006 Apr 7;342(2):647-54.
  •    Yousefi R, Imani M, Kabudanian-Ardestani S, Saboury A, Gheibi N, Ranjbar B, Human Calprotectin: Effect of Calcium and Zinc on its Secondary and Tertiary, Acta Biochim Biophys Sin. 2007 Oct;39(10):795-802. DOI: 10.1111/j.1745-7270. 2007.00343.x.
  •  Divsalar A, Saboury A, Yousefi R, Moosavi-Movahedi A, Mansori-Torshizi H, Spectroscopic and Cytotoxic Study of the Novel Designed Palladium Complexes: alpha-lactoglobulin and K562 as the Targets, Int J Biol Macromol. 2007 Mar 10;40(4):381-6. Epub 2006 Oct 4. DOI: 10.1016/j.ijbiomac.2006.09.015.
  •    Yousefi R, Kabudanian-Ardestani S, Imani M, Saboury A, Kariminia A,2007; ROS Induction by Human Calprotectin in K562 and the Reversal Effect of Vitamin E, Res J Bio Sci. 2:215-220. doi=rjbsci.2007.215.220.
  • Moosavi-Movahedi A, Amani M, Moosavi Nejad S, Hashemnia S, Ahmad F, Floris , Mura A, Rezaei-Tavirani M, Hakimelahi G, Saboury A, Yousefi R, Thermal dissection of lentil seedling amine oxidase domains by differential scanning calorimetry, Biosci Biotechnol Biochem. 2007 Jul;71(7):1644-9. DOI: 10.1271/bbb.70039.
  • Moosavi-movahedi A, Pirzadeh , Hashemnia S, Ahmadian S, Hemmateenejad B, Amani M, Saboury A, Ahmad F, Shamsipour M, Hakimelehi G, Tsai F, Yousefi R, Alijanvand H, Fibril formation of lysozyme upon interaction with sodium dodecyl sulfate at pH 9.2, Colloids Surf B Biointerfaces. 2007 Oct 15;60(1):55-61. DOI: 10.1016/j.colsurfb.2007.05.018.
  •     Yousefi R, Kabudanian-Ardestani S, Saboury A, Kariminia A, Zeinali M, Amani M, Investigation on the Surface Hydrophobicity and Aggregation Kinetics of Human Calprotectin in the Presence of Calcium, J Biochem Mol Biol. 2005 Jul 31;38(4):407-13. DOI:  10.5483/bmbrep.2005.38.4.407.
  •     Yousefi R, Saboury A, Ghadermarzi M, Moosavi-Movahedi A, Effects of cysteine on the inactivation of bovine liver catalase, Bull Kor Chem Soc. 2000; 21(6):567-570.

 

  •         Yousefi R. (2021) An overview of the important missions and achievements of US National Laboratories, The Journal of Science and Technology Policy Letters, In press.
  • 2)      Yousefi R, Moosavi Movahedi AA (2021) Science diplomacy as important opportunity for development of international scientific cooperation and strategic relations, Science Cultivation,   1-12, DOI: 20.1001.1.2008935.1400.12.1.2.8
  •        Nourazaran, M., Yousefi, R., & Moosavi-Movahedi, A. A. (2022). Effects of Vitamin D in Fighting COVID-19 Disease. Iranian Journal of Nutrition Sciences & Food Technology, 16(4), 121-130.
  •      The role of national laboratories and mission-oriented research institutes in the development of science and technology, (2021) Journal of Science, 2&3: 94-101.
  •     Oliaei M.S, Moosavi-Movahedi F, Yousefi R, Climate changes, destruction of the environment and their association with prevalence of coronavirus in human societies, Science Cultivation. 2020; 20.1001.1.2008935.1399.11.1.4.3
  •     Habibi-Rezaei M, Yousefi R, Important Strategies to Stop COVID-19: From Traditional Medicine to the Modern Vaccines, Science Cultivation. 2020; 42-53. (Review article).
  •     Moosavi-Movahedi F, Yousefi R, The new coronavirus: from prevention and treatment to the mechanism of proliferation in human body, Science Cultivation. 2020; 42-53. (Review article).
  •      Gorjizadeh N, Shahsavani MB, Moosavi-Movahedi F, Yousefi R, Analyzing structure, chaperone activity and aggregation of human αB-Crystallin in the presence of copper ions and glutathione, Modares Journal of Biotechnology, 2020 (Accepted for publication)/ (Research article).
  •      Shahsavani MB, Yousefi R,Application of Raman spectroscopy in protein structural analyses, Shaa Journal. 2020; 2(1) 13-28. (Review article).
  •     Salmanpour M, Yousefi R, Moosavi-Movahedi AA, Melatonin: an antioxidant product of good sleep, Science Cultivation. 2018; 7(2):107-115. (Review article).
  •    Javadi Arjmand, Yousefi R, An overview of criteria for family employment in some world universities, Science Cultivation. 2016; 5(2):77-80. (Review article)
  •     Akbarian M, Balalaei S, Yousefi R, Protein and peptide medicines: Prospective and future direction in Iran and globe, Razi: Pharmaceutical, Scientific and Social Journal. 2016; 26 (12): 1102-1119. (Review article).
  •     Moosavi Nezhad SZ, Dehghan Shahsaltened, Yousefi R, Moosavi-Movahedi AA, Destructive reactions in the body under the influence of synergistic factors: cigarette smoke, nervous stress and diabetes, Razi: Pharmaceutical, Scientific and Social Journal. 2011; 7: 663-668. (Review article).
  •    Yousefi R, Bagheri F, Salami M, Moosavi-Movahedi AA, Moosavi Nezhad SZ, The effect of cigarette smoke on diabetes mellitus, Razi: Pharmaceutical, Scientific and Social Journal. 2011; 9: 895-900. (Review article).
  •    Yousefi R, Salam M, Moosavi Nezhad SZ, Moosavi-Movahedi AA, Radioactive radiation of cigarette smoke and its association with cancer, Razi: Pharmaceutical, Scientific and Social Journal. 2011; 5: 458-464. (Review article).
  •    Jamalian A, Yousefi R, Moosavi-Movahedi AA, Effective medicines to quit smoking, Razi: Pharmaceutical, Scientific and Social Journal. 2011; 10: 945-954. (Review article).
  •   Moosavi Nezhad SZ, Seifi Abolhasani M, Dehghan Shahsaltaneh M, Rahmati F, Yousefi R, Salami M, Moosavi-Movahedi AA, Absorption and release of nicotine in nicotine products and drugs and their effects on the body, Razi: Pharmaceutical, Scientific and Social Journal. 2011; 6: 566-575. (Review article).
  •    Jadaliha M, Yousefi R, Moosavi-Movahedi AA, Cigarette smoke, genomic destruction and its transmission to the next generation, Razi: Pharmaceutical, Scientific and Social Journal. 2010; 20: 211-218. (Review article).
  •   Alizadh N, Yousefi R, Moosavi Nezhad SZ, Moosavi-Movahedi AA, Electronic cigarettes, Razi: Pharmaceutical, Scientific and Social Journal. 2010; 9: 851-858. (Review article).
  •  Moosavi-Movahedi, Poorsasan N, Salami M, Yousefi R, Role of nutrition and medicinal plants in modulating the damages from cigarette smoke, Razi: Pharmaceutical, Scientific and Social Journal. 2009; 20: 118-126. (Review article).
  •   Yousefi R, Atri MS, Salami M, Niasari-Naslaji A, Saboury AA, Moosavi-Movahedi AA, Anti-cancer and antimicrobial properties of milk proteins and peptides, Razi: Pharmaceutical, Scientific and Social Journal. 2011; 11: 1132-1137. (Review article).
  •   Yousefi R, Izadi M, Niazi A, Salami M, Moosavi-Movahedi AA, Effect of probiotics on human health, Razi: Pharmaceutical, Scientific and Social Journal. 2011; 30: 327-336. (Review article).
  •   Yousefi R, Shahsavani MB, Khalafi-Nezhad A, Moosavi-Movahedi AA, Role of nano carriers in the specific delivery of anticancer drugs, Razi: Pharmaceutical, Scientific and Social Journal. 2011; 31: 507-514. (Review article).
  • Yousefi R, Moosavi-Movahedi AA, Nicotine as precursor for carcinogens in the body, Razi: Pharmaceutical, Scientific and Social Journal. 2007; 10: 918-927. (Review article).

 

Editorial Activities:

  • Guest Editor of the Special Issue on Protein misfolding and aggregation in cataract disorders" Biochemistry (Moscow), February 2022, Volume 87, issue 2.
  • Guest Editor of the Special Issue on Covid-19 Pandemic: Epidemiology, Ways of Coping and Prevention, Diagnosis, Treatment Strategies Mechanism of Action and Log-Term Complications. It will be published by Iranian Journal of Science & Technology, Transaction A in September 2022.
  • Editorial board member of Biochemistry (Moscow).
  • Academic editor of PLOS One.
  • Editorial board member of Iranian Journal of Science & Technology, Transaction.

Msc Students

  • Ahmadreza Ghaffari
  • Aref Baharvand
  • Atefeh Salehi
  • Mahdi Hemmati

PhD Students of Biochemistry

  • Leila Rezaei Somee
  • Mahsa Heidari
  • Zahra Bahrami
  • Zahra Mirazaei
  • Sarvenaz Moradi
  • Mohamadreza Abolhasan
  • Mohamadali Ahmadi

 

Postdoc/Research Associate

 

 

Graduate Students of Protein Chemistry Laboratory (PCL)

Name

Thesis 

Year
Farid Nasiri/ PhD Dissecting the role of p.R107L Mutation on Structure, Stability, Chaperone Activity and Fibrillation Properties of Human αB-Crystallin. 2023
Mohammad Bagher Shahsavani/ PhD Investigating the effect of (p.Ter207extArgArgTer),(p.Ter207extHisHisTer) and (p.Ter207extLysLysTer)mutations on the structure, stability and amyloid 2023

Mehrnaz Hosseini Jafari

/ PhD

 Structural and functional characterization of D109G human recombinant αB-Crystallin 2023
Ravieyeh Muslem Albaghlani / PhD Optimizing expression, purification, structural and functional assessments of a novel dimeric incretin (GLP-1cpGLP-1) 2023
Sayed Hossein Khaleghinezhad/ PhD Investigating the role of double mutations R12C/P20R, and R12C/R69C on structure, chaperone-like activity, and amyloidogenic properties of human αB-crystallin 2022

Fatemeh Khosravi/ PhD

Expression and Production of Human Recombinant Proinsulin with αB-crystallin as Fusion Protein

2022

Anis Barati/ PhD

Effects of Tau and Amyloid beta on the Expression of Neuroinflammation Genes in Drosophila Melanogaster Models of Alzheimer's Disease and the Modulatory Effect of β-D Mannuronic Acid (M2000)

2022

Samaneh Ahmadi/ M.Sc.

Optimizing the expression, purification and bioactivity of the anti-diabetic peptide liraglutide

2021

Mohmmad Mehdi Ghanbarnezhad/ M.Sc.

Expression, Purification, Structural Analyses and Biological Activity Assessment of New Analogue of Anti-Diabetic Drug Exenatide

2021

Parto Nasiri/ M.Sc.

Role of R157H Mutation on the Structure, Stability, Chaperone Activity and Amyloidogenic Properties of Recombinant Human αB-crystallin

2020

Mehrnaz Haghi/ M.Sc.

Effect of Tau and amyloid beta on the expression of autophagy genes in Drosophila Melanogaster models of Alzheimer,s diseases and investigation of inhibitory role of thymoquinone in Tau aggregate formation

2020

Masoumeh Yousefi/ M.Sc.

Role of oxidative stress on the structure, stability, chaperone activity and amyloidogenesis of human R11C and R69C mutant αB-crystallins

2020

Mousa Amirahmadi/ PhD

The effect of concurrent training and antioxidant supplementation on the factors associated with ocular lens opacity in diabetic rats

2019

Maryam Ghahramani/ PhD

role of mutations R11C, P20R, R69C, D109H and A171T in structure, stability, chaperone activity and amyloidogenic properties of recombinant human aB crystallin

2019

Mohsen Akbarian/ PhD

Study on the role of mutations (T30R and K29R/T30R) on structure, stability and amyloidogenesis of human insulin

2019

Mahtab Hafizi Yazdabadi/ M.Sc.

Study on the effects of D109A mutation on the structure, stability, chaperone activity and amyloidogenesis of Human αB-crystallin

2019

Paria Dizgooni/ M.Sc.

Protective effect of pyruvate and lipoic acid against Riboflavin-mediated photo-damaging of human recombinant a-crystallin

2018

Fatemeh Hasani Nezhad/ M.Sc.

Study on the Inhibitory effect of novel curcumin- based heterocycle compounds against a-Glucosidase and a-amilase enzymes

2018

Nooshin Gorjizadeh/M.Sc.

Study of the effect of Copper ions on structure, chaperone activity and aggregation of human mutant αB -Crystallin R12C in the presence of ascorbic acid and glutathione

2018

Pezhman Ashoo/ M.Sc.

 

Study on anticancer properties of dimeric donor-acceptor platinum complexes and assessment of their interaction with DNA and human serum albumin

2018

Kazem Khoshaman/ PhD

Study on the role of R54X mutations on the structure, stability, chaperone activity, amyloidogenesis and pattern of disulfide bond formation in recombinant human αA-Crystallin

2017

Afrooz Anbaraki/ M.Sc.

Study on the riboflavin-mediated photo-damaging of human recombinant αA-Crystallin and total soluble lens proteins in the presence of natural antioxidants

2016

Tanaz Sadeghian/ M.Sc.

Study on structure, chaperone activity and allergenicity of peroxynitrite-modieied beta-casein of bovine milk

2016

Maryam Kianpour/ M.Sc.

Study of the inhibitory properties of Curcumin and Carnosine against damaging reactions between methylglyoxal and lens crystallins

2016

Sogand Sasanmoghadam/ M.Sc.

 

Study on the role of peroxynitrite and methylglyoxal on structure, aggregation and functional properties of lens crystallins

2016

Zeinab Moafian/ M.Sc.

The protective role of acetylation on structure and aggregation properties of lens crystallins in the presence of homocysteine thiolactone

2016

Sajjad Javadi/ M.Sc.

Effect of Carnosine and Aspirin on structure and aggregation properties of lens proteins in the presence of dehydroascorbic acid

2016

Homa Ehsan/ M.Sc.

Synthesis and antibacterial assessment of silver nanoparticles in the presence of caseins glycopeptides

2015

Sara

Shahidpour/ M.Sc.

Study on the inhibitory properties of carbohydrate containing pyrimidine- fused heterocycles against α- glucosidase and assessment of their interaction with β- lactoglobulin

2015

Afsoon Yousefi/ M.Sc.

Study on the inhibitory properties of Curcumin-based Pyrano [2,3.d]Pyrimidine derivatives against α-glucosidase and assessment of their interaction with β-lactoglobulin

2014

Maryam Ghahramani/ M.Sc.

The impact of copper ion on structure, chaperone activity and amyloidogenic properties of peroxynitrite-modified human AlphaA-crystallin and investigation on the effect of calcium ion on aggregation of peroxynitrite-modified bovine lens crystallins

2014

Zohreh Fereidouni Roodbali/ M.Sc.

Study on anticancer activity of poly phosphine cationic platinum (II) complexes and assessment of their interaction with DNA and serum albumin

2014

Leila Ferdowsi/ M.Sc.

 

Study on structure, chaperone activity and allergenicity of glycated and non-glycated beta-caseins

2014

Mohadeseh Ragerdi Kashani/ M.Sc.

Study on the role of R12C mutation on structure and amyloidogenic properties of human alpha-B crystallin

2014

Sadaf Saba/ M.Sc.

Study on the role of r12c mutation on structure and amyloidogenic properties of human alpha-a crystallin

2014

Sara Zafaranchi Zadeh Moghadam/ M.Sc.

Study on the role of calcium binding on structure and amyloidogenic properties of glycated and non-glycated gamma crystallin

2014

Shahrzad Hajjari/ M.Sc.

Study on the effect of homocysteine thiolactone on structure and fibrillation of acetylated and non- acetylated gamma-crystallin

2013

Behnaz Taheri/ M.Sc.

Study on the role of acetylation on structure and amyloidogenic properties of insulin hormone

2013

Zahra Toobaei/ M.Sc.

Study on the inhibitory properties of some new poly-hydroxyl functionalized acridines against yeast and mouse alpha-glucosidases and determination of their binding parameters on beta-lactoglobulin

2013

Roghayeh Mohammadi/ M.Sc.

Anticancer activities and DNA/protein binding properties of a novel series of cyclo-methyl-platinum (II) complexes

2013

Sara Amirghofran/ M.Sc.

Study on the structure and amyloidogenesis of glycated and non-glycated crystallins

2013

Zahra Ghazanfari/ M.Sc.

Comparative study on structure, chaperone activity and amyloidogenesis of camel and bovine alpha-crystallins

2013

Zahra Pouryasin/ M.Sc.

Study on anticancer activities and DNA/protein binding properties of a novel series of platinum(IV)- bismuth(III) complexes

2013

Parnian Alavi/ M.Sc.

Role of non-enzymatic glycation on structure and amyloidogenic properties of reduced and non-reduced glucosylated insulin adducts

2013

Mehrnaz Jamshidi/ M.Sc.

Study on anticancer activity of a novel series of cationic platinum (II) complexes and assessment of their interaction with DNA and serum albumin

2013

Sima Khazaei/ M.Sc.

Role of homocysteinylation on structure, chaperone activity and amyloidogenic properties of alpha-crystallin and total lens proteins

2012

Mohammad Hossein Mehraban/ M.Sc.

Study on the inhibitory properties of novel pyrimidine-fused heterocycles against α-glucosidase and the thermodynamic characterization of their binding interactions to beta-lactoglobulin

2012

Hojjat Khalili-Hezarjaribi/ M.Sc.

Effect of Novel Derivatives of Phenyl Benzothiazolium Salt on Non-Enzymatic Glycation and Chaperone Activity of Alpha-Crystallin

2011

Shima Jalili/ M.Sc.

Effect of homocysteine thiolactone on structure and amyloidogenic properties of insulin

2011

Sadaf Aghevlian/M.Sc.

Study of gold nanoparticles cytotoxicity and photoactivation effect of gold nanoparticles, Thioguanine gold nanoparticles, Thioguanine and Cisplatin on cancer cell

2011

 

 

Advanced Protein Biochemistry

The Purpose of the Curriculum  

The polymer structure consisting of twenty monomeric building units of proteins (twenty standard alpha-amino acids) and their subsequent folding into a fine three-dimensional structure allows these biomolecules to perform a wide range of biological tasks, from catalytic roles to participation in signaling pathways. In addition to the structural and functional diversity, proteins and their building blocks have been widely used in medical fields and various industries. Understanding this relationship helps biochemistry students to deepen their basic knowledge in the field of protein structure and function, and to learn the important and diverse applications of these biomolecules in different fields. The purpose of this course is to teach graduate students the connection between the principles and scientific rules governing the structure and function of proteins and their practical and applied areas.

 Syllabus Details

(1) Introduction of bio elements in the periodic table, important functional groups, isomerism and non-covalent interactions in biomolecules. (2) Standard alpha-amino acids and biological importance/medical applications of their derivatives. (3) Peptide bond, Ramachandran plot and introduction of medical and industrial applications of peptidase enzymes. (4) Description of the traditional and new methods in determining the primary structure of proteins. (5) Scientific bases of the formation of protein secondary structures and the methods of studying these regular structural elements. (6) Stability of proteins, mechanism and thermodynamics of protein folding. (7) The mechanism of protein aggregation/fibrillation, amyloid diseases and chaperone systems.(8) Different protein families and super families based on biological functions and structural elements. (9) The importance of filamentous and globular proteins in living systems and their medical and industrial applications. (10) The role of proteins in signaling; introducing the related pathological disorders and therapeutic solutions. (11) Structure and functions of antibodies, production methods and their medical applications. (12) Medical and industrial applications of enzymes and enzyme inhibitors. (13) Therapeutic applications of protein and peptide based hormones. (14) The key role of proteins and peptides in immune system.

Reading Resources:

1) Gray Walsh, Proteins Biochemistry and Biotechnology, John Wiley & Sons, 2002.

2) Jack Kyte, Structure in Protein Chemistry, Garland Science, 2006.

3) David Whitford, Proteins: Structure and Function, Wiley; 1st edition, 2005.

4) Dev Bukhsh Singh,  Timir Tripathi, Frontiers in Protein Structure, Function, and Dynamics, Springer Nature Singapore Pte Ltd, 2020.

Evaluation Methods

- Assignments, semester projects, mid-term and final exams.

Methods in Biochemistry

The Purpose of the Curriculum

The purpose of this course is to transfer the basic knowledge of important and widely applied methods that are often used in biochemistry research to master's and doctoral students of biochemistry.

 Syllabus Details

(1) Analytical Methods- Protein, nucleic acid and enzyme analyses. (2) Chromatography Methods - In this section, the principles of size exclusion chromatography (SEC), ion exchange chromatography, hydrophobic interaction chromatography, reverse phase chromatography, affinity chromatography, chromatofocusing, and HPLC/FPLC will be taught. (3) Electrophoresis Methods- In this section, one-dimensional electrophoresis (1-DE) including different types of polyacrylamide gel electrophoresis such as reducing and non-reducing SDS-PAGE⸲ Urea PAGE⸲ native gel electrophoresis, also isoelectrofocusing (IEF) and two-dimensional electrophoresis are taught and discussed along with their important applications. (4) Spectroscopy Methods- In this part, the principles and application of different spectroscopic methods such as UV-Vis absorption spectroscopy, Fluorescence, infrared spectroscopy (IR), Raman spectroscopy, circular dichroism (CD), dynamic light scattering (DLS), mass spectrometry (MS) and nuclear magnetic resonance (NMR) are taught and discussed. (5) Immunochemistry Methods- Principles and important applications of radioimmunoassay (RIA)⸲ western blot and ELISA will be taught. (6) Microscopic Methods- Principles and a number of applications of scanning electron microscope (SEM), transmission electron microscope (TEM), atomic force microscope (AFM) and fluorescence microscope in biochemistry researches are discussed. (7) Thermal Analysis Methods- Principles and applications of differential scanning calorimetry (DSC) and thermal gravimetric analysis (TGA) will be taught. (8) Gene Manipulating Techniques- RNA and DNA extraction, PCR, qPCR and gene cloning.

Evaluation Methods

- Assignments, semester projects, mid-term and final exams.

Reading Resources:

1. Claus Urbanke , Jim Hoggett , Albert Jeltsch , Biochemical Methods: A Concise Guide for Students and Researchers, Wiley-VCH Verlag GmbH, 2003. ISBN: 978-3-527-30299-4.

2. Keith Wilson (Editor), John Walker (Editor), Principles and Techniques of Biochemistry and Molecular Biology, Cambridge University Press; 7th edition, 2013. Online ISBN: 9780511841477.

Advanced Topics in Biochemistry

The Purpose of the Curriculum

The purpose of this course is basically to acquaint graduate students with the important fact of how the principles and concepts of biochemistry have been manifested in various scientific and applied fields. After completing this course, students are prepared to enter important scientific and applied fields in which biochemistry plays a prominent role

 Syllabus Details

Biochemistry of cell cycle and programmed cell death

  • Introducing the molecular factors involved in the different stages of cell cycle and its relationship with the DNA repair system and programmed cell death (apoptosis).
  • Elucidating the role of molecular factors involved in the intrinsic and extrinsic pathways of apoptosis.
  • Describe the practical methods of studying programmed cell death.
  • Description of the structure and function of proteasome and the pathway of destruction of damaged proteins and viral/bacterial proteins.

Biochemistry of the immune system

  • Structure⸲ function⸲ application and diversity of antibodies and other proteins of the immunoglobulin superfamily.
  • Introducing the structure and function of the complement system and the associated genetic diseases.
  • The structure and function of cytokines and their receptors.
  • Description of cytokine signaling pathways and medical applications of cytokines.

Biochemistry of neurotransmission

  • Describing the molecular details of nerve cells and neural connections.
  • Structure⸲ function and metabolism of neurotransmitters.
  • Biochemical bases of the functioning mechanism of the five senses.
  • Investigating the structure and function of important molecular factors involved in the induction of sleep and wakefulness processes.

Biochemistry of hormones

  • Introduction of the structure⸲ function and biosynthesis of water-soluble and fat-soluble hormones.
  • Diseases of hormonal origin and therapeutic applications of hormones.
  • Introducing the structure and function of hormone-like substances.

Biochemistry of blood

  • Classification and introduction of the structure and function of the plasma proteins.
  • Biochemistry of blood coagulation cascade and description of the structure and mechanism of action of anticoagulant agents.
  • Introducing the biochemical mechanism of a number of the diagnostic tests using blood molecular markers.

Proteomics

  • Applications of two-dimensional (2D) electrophoresis and mass spectrometry in the quantitative and qualitative identification of proteins and peptides.

 Applied Biochemistry

The important applications of biochemistry in the fields of medicine, agriculture and industry.

 Reading Resources:

- Puthalakath H.,   Christine J. Hawkins, Programmed Cell Death Methods and Protocols, Humana Press, 2016.  
- Sompayrac,L.M., How the Immune System WorksWiley-Blackwell; 6th edition, 2019.
- McIlwain H., Biochemistry of the Central Nervous System, Churchill Livingstone; 4th Revised edition, 1971.
- Rickenberg, H. V., Biochemistry of hormones. London: Baltimore, Butterworths, 1974. 
- Nawin C. Mishra, Introduction to Proteomics: Principles and Applications, Wiley; 1st edition, 2010.
- Garg,G.P.  Industrial Biochemistry, Discovery Publishing House, 2012.

 

Proteins Engineering

(Suggested course for the future semesters)

The Purpose of the Curriculum

Today, protein engineering products are very diverse and they are used in various industries such as pharmaceutical, health, chemical, food, paper, leather and cosmetic industries. The general goal of this course is to acquaint graduate students of biochemistry with the different stages of engineering and production of proteins and peptides. The specific objectives of this course are as following: (1) Teaching common methods and strategies in protein and peptide engineering. (2) Teaching the methods of optimizing the production of recombinant proteins and peptides. (3) Introducing important solutions for the purification of recombinant proteins and peptides and peptides. (4) Introducing pharmaceutical forms and mechanism of action of medicinal proteins and peptides.

Syllabus Details

(1)  Importance, advantages and disadvantages of producing recombinant proteins in different hosts. (2)  Introduction of expression vectors and important hosts for the production of recombinant proteins and peptides. (3)  Optimizing genetic codons and discussing the principles of cloning and site directed mutagenesis.  (4) Important challenges and solutions in the effective expression of proteins and peptides (5) Important solutions to overcome the challenge of instability of recombinant proteins and peptides. (6) Introduction of promoters and leader sequences in directing expression and details of secretory pathways in prokaryotic and eukaryotic cells. (6)  Important strategies for optimizing the expression of recombinant proteins and peptides as well as different methods of digesting the expression host cells. (7) Important strategies for the production and purification of recombinant proteins and peptides. (8) DNA sequencing and identification of recombinant proteins and peptides. (9)  Folding and refolding methods of recombinant proteins with the aim of obtaining a bioactive final product. (10) Classification of the medicinal proteins and peptides based on the mechanism of action and presenting important examples of the use of recombinant proteins and peptides in the therapeutic fields.

Reading Resources:

1. Kakoli Bose, Textbook on Cloning, Expression and Purification of Recombinant Proteins,  Springer Singapore; 1st ed. 2022 edition.

 2. Olga Iranzo, Ana Cecília Roque (Editor),  Peptide and Protein Engineering, From Concepts to Biotechnological Applications, Humana Press; 1st ed. 2020.

3.  Katja M. Arndt,  Kristian M. Müller (Editor), Protein Engineering Protocols, Humana Press, Totowa NJ, 2007

4. Sheldon J. Park,  Jennifer R. Cochran,  Protein Engineering and Design, CRC Press, 2009

5. M. Ramya, P. Ponmurugan, Protein Engineering, Alpha Science International Ltd. 2015.

6. Yury E. Khudyakov, Medicinal Protein Engineering, CRC Press, 2019.

Evaluation Methods

- Assignments, semester projects, mid-term and final exams.

 

 

​Previous Teaching Experience (2008-2021; Shiraz University)

BSc: Biochemistry-I (Structure & Function), Biochemistry-II (Metabolism), Molecular Biology.

MSc: Methods in Biochemistry, Advanced Molecular Biology, Proteomics, Immunology.

PhD: Advanced Topics in Protein Biochemistry, Methods in Biochemistry, Nucleic Acid Structure, Proteomics.

 

Managing Activities

  • Director of the Central Laboratory of Shiraz University- 2016-2021.
  • Consultant Professor of the Scientific Association of Shiraz University Biology Students 2013-2015.
  • Scientific Chair of 1st National Conference on Protein and Peptide Sciences (PPS1)- 2013.
  • Scientific Chair of 3rd National Conference on Protein and Peptide Sciences (PPS3)- 2017
  • Scientific Chair of the first National Conference of Laboratory Equipment and Measurement Technologies-  2020.
  • Responsible for commemorating the National Laboratory Day and the Birthday of Hakim Ismail Jarjani- 2021.

 

 

 

Awards and honors

 

Recognized as top professor in the educational activity in Shiraz University- 2012

Recognized as a top researcher in Shiraz University- 2013

Honored consultant professor of the scientific association of biology students- 2014

Recognized as a top researcher in Shiraz University- 2018

 

 

 

 

Industrial cooperation

 

Protein Chemistry Laboratory (PCL) is ready to cooperate with various industries by taking advantage of long experience and knowledge in the field of purification⸲ structural and functional studies of proteins and peptides. Our team members possess proficiency in diverse methods of producing and purifying proteins and peptides on a laboratory scale, both from natural sources and using recombinant DNA technology in the bacterial host system. Furthermore, we possess a comprehensive understanding of the structural, stability, and functional assessments of proteins and peptides. We welcome the opportunity to engage in industrial cooperation and look forward to sharing our knowledge and skills to advance the goals of our partners in the industry sector. We welcome the opportunity to collaborate on joint scientific-technological projects, as well as implement industry-proposed projects, with the participation of doctoral students and postdoctoral researchers. Research proposals related to the industrial sector are also in compliance with the regulations of University of Tehran (UT). Therefore, we encourage proposals from both pharmaceutical and non-pharmaceutical industries that are interested in this type of cooperation. Working together with our partners to attain mutual goals is something we are eagerly anticipating