Name: Arefeh Seyedarabi
Appointment: Assistant Professor of Biochemistry and Structural Biology
Office Address: Institute of Biochemistry and Biophysics,
University of Tehran P.O.Box 13145-1384 Tehran, Iran
Telephone No.: +98-21 66956974
Fax: 0098 21 66404680
March 2012-present: Assistant Professor of Biochemistry, Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran
Structure-function studies of proteins involved in health and disease using various biochemical and biophysical techniques.
Jan 2011-2012: Senior Research Associate, Institute of Structural and Molecular Biology, University College London (Funded by British Heart Foundation)
My second postdoctoral research associate position was with Dr Snezana Djordjevic working on the structural and functional analyses of neuropilin-1 and neuropilin-2 interactions with VEGF which involved thermodynamic and kinetics experiments, X-ray crystallography, cell-free and cell-based assays as well as in vivo studies.
May 2007- 2010: Postdoctoral Research Assistant, School of Biological and Chemical Sciences, Queen Mary University of London (in collaboration with University of Kent, UK and Funded by BBSRC)
My postdoctoral research assistant position was with Professor Richard Pickersgill studying the substrate recognition and reaction mechanism of methyltransferases from the cobalamin (Vitamin B12) biosynthetic pathway (in collaboration with Professor Martin Warren, University of Kent). I was able to solve the structures of the following methyltransferases; CobF, CobM and CobL (C- terminal domain) in complex with S-adenosyl homocysteine (the demethylated form of the methyl donor S- adenosyl methionine). I have a first author publication (in Acta Cryst F) and will have more publications pending on the results obtained from this study.
Oct. 2003- 2007: PhD in Structure-function studies of proteins involved in infection, School of Biological and Chemical Sciences, Queen Mary University of London (in collaboration with University of Tokyo, Tokyo, Japan)
The focus of my study was on proteins belonging to the human pathogenic bacteria Listeria
monocytogenes and in particular Shigella flexneri (in collaboration with Professor Chihiro Sasakawa, University of Tokyo) and also the plant pathogenic bacteria Bacillus subtilis. I solved the structure of the dimeric form of Shigella flexneri IpaH9.8 E3 ubiquitin ligase and showed for the first time that its human substrate is U2AF35. In the study of Bacillus subtilis, I solved a number of structures involving Michaelis complexes of pectate lyase bound to different polygalacturonates to unravel the reaction mechanism of the enzyme as well as carrying out ITC experiments to unravel the binding affinity and stoichiometry of hexagalacturonate and pectate lyase. I have first author publications in FEBS Letters and Biochemistry from the studies on Shigella flexneri IpaH9.8 and Bacillus subtilis pectate lyase, respectively.
Sept. 2000-June 2003: First Class Honours BSc degree in Genetics and Microbiology,School of Biological Sciences, Queen Mary University of London (obtained the Westfield Trust Prize for Outstanding Academic Achievement)